Free Peptide Reconstitution Calculator

Calculate accurate peptide dosing, syringe units, and reconstitution volumes for 30+ peptides. Select your peptide, enter your values, and get instant results with a visual syringe guide.

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Peptide Reconstitution Calculator

Select a peptide or enter custom values. All calculations are performed locally in your browser.

Step 1: Select Peptide

Step 2: Peptide Vial Strength (mg)

Step 3: Bacteriostatic Water Added (mL)

Step 4: Desired Dose

mcg

Your Results

Concentration

2.5 mg/mL

= 2500 mcg/mL

Volume to Draw

0.10 mL

Syringe Units (U-100 insulin syringe)

10.0 units

100 units = 1 mL on a standard U-100 insulin syringe

Doses Per Vial

20 doses

U-100 Insulin Syringe

50u

100u

10.0 units

0.10 mL

Summary: Add 2 mL of bacteriostatic water to your 5mg vial. To get a 250 mcg dose, draw 0.10 mL (10.0 units) on a U-100 insulin syringe. This vial will provide 20 doses at this dosage.

For research purposes only. This calculator is a mathematical tool and does not constitute medical advice. Always consult with a qualified healthcare professional before using any peptide.

How to Use This Calculator

Select Peptide

Choose from our database of 30+ peptides or enter custom values. Each peptide comes with preset typical dose ranges and common vial sizes.

Enter Vial Strength

Select your vial's total peptide content in milligrams (mg). Use the preset buttons or enter a custom amount.

Add Water Amount

Enter how much bacteriostatic water you added (or plan to add) to the vial in milliliters (mL).

Read Your Dose

Get your exact concentration, volume to draw, syringe units, and doses per vial -- plus a visual syringe indicator.

How Peptide Reconstitution Works

What is Reconstitution?

Peptides are shipped as a lyophilized (freeze-dried) powder because the dry form is significantly more stable than a liquid solution. Reconstitution is the process of adding bacteriostatic water (BAC water) to this powder to create an injectable solution. The amount of water you add determines the concentration of the final solution, which directly affects how much liquid you need to draw for each dose.

The Math Behind It

The core calculation is simple division:

Concentration = Vial Strength / Water Volume

Volume to Draw = Desired Dose / Concentration

Syringe Units = Volume (mL) x 100

Why Bacteriostatic Water?

Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits bacterial growth. This allows you to draw from the same vial multiple times over several weeks without contamination. Regular sterile water lacks this preservative and should only be used for single-use preparations.

Proper Technique

When adding water to a peptide vial, aim the stream of water down the inside wall of the vial rather than directly onto the powder. This gentle approach prevents damaging the peptide molecules. After adding water, gently swirl the vial -- never shake it. Most peptides dissolve within seconds to a couple of minutes.

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Peptide Dosing Quick Reference

Typical dose ranges from published research. Always verify dosing with a qualified healthcare professional.

Peptide	Category	Typical Dose	Frequency	Route	Calculator
BPC-157	Healing & Recovery	250 mcg - 500 mcg	1-2x daily	Subcutaneous injection	Open
TB-500	Healing & Recovery	2 mg - 5 mg	2x per week (loading), 1x per week (maintenance)	Subcutaneous or intramuscular injection	Open
Semaglutide	GLP-1 / Weight Management	250 mcg - 2.40 mg	1x per week	Subcutaneous injection	Open
Tirzepatide	GLP-1 / Weight Management	2.50 mg - 15 mg	1x per week	Subcutaneous injection	Open
CJC-1295 (with DAC)	Growth Hormone Secretagogues	1 mg - 2 mg	1-2x per week	Subcutaneous injection	Open
CJC-1295 (no DAC) / Mod GRF 1-29	Growth Hormone Secretagogues	100 mcg - 300 mcg	2-3x daily	Subcutaneous injection	Open
Ipamorelin	Growth Hormone Secretagogues	200 mcg - 300 mcg	2-3x daily	Subcutaneous injection	Open
GHRP-6	Growth Hormone Secretagogues	100 mcg - 300 mcg	2-3x daily	Subcutaneous injection	--
GHRP-2	Growth Hormone Secretagogues	100 mcg - 300 mcg	2-3x daily	Subcutaneous injection	--
GHK-Cu	Healing & Recovery	1 mg - 2 mg	1x daily	Subcutaneous injection or topical	--
Epithalon	Longevity	5 mg - 10 mg	1x daily for 10-20 days	Subcutaneous injection	--
PT-141 (Bremelanotide)	Sexual Health	1 mg - 2 mg	As needed (max 1x per 24 hours)	Subcutaneous injection	Open
Melanotan II	Tanning / Sexual Health	250 mcg - 1 mg	1x daily (loading), then maintenance	Subcutaneous injection	Open
AOD-9604	Weight Management	300 mcg - 600 mcg	1x daily	Subcutaneous injection	--
HGH Fragment 176-191	Weight Management	250 mcg - 500 mcg	1-2x daily	Subcutaneous injection	--
Sermorelin	Growth Hormone Secretagogues	200 mcg - 500 mcg	1x daily (before bed)	Subcutaneous injection	--
Hexarelin	Growth Hormone Secretagogues	100 mcg - 200 mcg	2-3x daily (max 4 weeks)	Subcutaneous injection	--
Selank	Nootropic / Anxiolytic	250 mcg - 500 mcg	1-3x daily	Intranasal or subcutaneous	--
Semax	Nootropic / Neuroprotective	200 mcg - 600 mcg	1-3x daily	Intranasal	--
Kisspeptin-10	Hormonal / Reproductive	50 mcg - 100 mcg	1-2x daily	Subcutaneous injection	--
DSIP (Delta Sleep-Inducing Peptide)	Sleep / Recovery	100 mcg - 300 mcg	1x daily (before bed)	Subcutaneous or intranasal	--
Gonadorelin	Hormonal / Reproductive	100 mcg - 500 mcg	2-3x per week	Subcutaneous injection	--
Tesamorelin	Growth Hormone Secretagogues	1 mg - 2 mg	1x daily	Subcutaneous injection	--
IGF-1 LR3	Growth / Muscle	20 mcg - 100 mcg	1x daily (training days)	Subcutaneous or intramuscular injection	--
Follistatin 344	Growth / Muscle	100 mcg - 300 mcg	1x daily for 10-30 days	Subcutaneous injection	--
GLP-1 (Generic)	GLP-1 / Weight Management	500 mcg - 3 mg	Varies by specific analog	Subcutaneous injection	--
Retatrutide	GLP-1 / Weight Management	500 mcg - 12 mg	1x per week	Subcutaneous injection	Open
NAD+	Longevity	50 mg - 500 mg	1-3x per week	Intravenous (IV) or subcutaneous	--
Dihexa	Nootropic / Neuroprotective	10 mg - 20 mg	1x daily (oral or subQ)	Oral (sublingual) or subcutaneous	--
SS-31 (Elamipretide)	Longevity / Mitochondrial	5 mg - 40 mg	1x daily	Subcutaneous injection	--

Individual Peptide Calculators

Dedicated calculator pages with peptide-specific dosing information, FAQs, and pre-loaded defaults.

BPC-157

Healing & Recovery

250 mcg - 500 mcg | 1-2x daily

TB-500

Healing & Recovery

2 mg - 5 mg | 2x per week (loading), 1x per week (maintenance)

Semaglutide

GLP-1 / Weight Management

250 mcg - 2.40 mg | 1x per week

Tirzepatide

GLP-1 / Weight Management

2.50 mg - 15 mg | 1x per week

CJC-1295 (with DAC)

Growth Hormone Secretagogues

1 mg - 2 mg | 1-2x per week

CJC-1295 (no DAC) / Mod GRF 1-29

Growth Hormone Secretagogues

100 mcg - 300 mcg | 2-3x daily

Ipamorelin

Growth Hormone Secretagogues

200 mcg - 300 mcg | 2-3x daily

PT-141 (Bremelanotide)

Sexual Health

1 mg - 2 mg | As needed (max 1x per 24 hours)

Melanotan II

Tanning / Sexual Health

250 mcg - 1 mg | 1x daily (loading), then maintenance

Retatrutide

GLP-1 / Weight Management

500 mcg - 12 mg | 1x per week

How to Read an Insulin Syringe

Understanding U-100 insulin syringe markings is essential for accurate peptide dosing.

0.3 mL Syringe (30 units)

Best for small doses. Each small tick = 0.5 units. Ideal when drawing less than 30 units. Most precise for microgram-range peptides.

0.5 mL Syringe (50 units)

Good middle ground. Each small tick = 1 unit. Commonly used for most peptide injections where doses fall between 10-50 units.

1 mL Syringe (100 units)

For larger volumes. Each small tick = 2 units. Used when drawing higher-volume doses or when using dilute reconstitutions.

Key conversion: On any U-100 insulin syringe, 100 units = 1 mL. So 10 units = 0.1 mL, 50 units = 0.5 mL, and so on. This is why our calculator shows both mL and units -- they are directly convertible.

Read the full syringe guide →

Safety and Best Practices

Always Use Sterile Technique

Swab vial tops with alcohol before each draw. Use a new needle for each injection. Never touch the needle to any non-sterile surface. Wash hands thoroughly before handling supplies.

Store Properly

Unreconstituted peptides can be stored at room temperature or refrigerated. Once reconstituted, always refrigerate at 2-8 C. Never freeze reconstituted solutions. Keep away from light.

Respect Expiration Dates

Most reconstituted peptides are stable for 14-30 days when refrigerated. Mark the reconstitution date on your vial. Discard any solution that appears cloudy, discolored, or has particles.

Start Low and Go Slow

If you are new to a peptide, always start at the lower end of the typical dose range. Assess your response over several days before increasing. This minimizes the risk of adverse effects.

Educational Resources

Reconstitution Guide

Step-by-step guide to reconstituting peptides safely and correctly.

Dosing Chart

Comprehensive reference table for all 30+ peptides with dose ranges.

Syringe Guide

Learn to read U-100 insulin syringe markings accurately.

BAC Water Guide

Everything about bacteriostatic water: what it is, how to use it, and alternatives.

Storage Guide

How to store peptides before and after reconstitution for maximum stability.

Full Peptide Database

Browse all 30+ peptides with categories, routes, and dose ranges.

Frequently Asked Questions

Reconstitution is the process of adding a liquid (typically bacteriostatic water) to a lyophilized (freeze-dried) peptide powder to create an injectable solution. The peptide comes as a dry powder in a sealed vial, and you add a specific amount of water to dissolve it before use.

Bacteriostatic water (BAC water) is sterile water that contains 0.9% benzyl alcohol as a preservative. The benzyl alcohol prevents bacterial growth, allowing the reconstituted peptide solution to remain safe for multiple uses over several weeks. It is the standard diluent for peptide reconstitution.

Divide the total peptide amount in the vial (in mg) by the volume of water added (in mL). For example, a 5mg peptide vial reconstituted with 2 mL of BAC water gives a concentration of 2.5 mg/mL (or 2,500 mcg/mL).

A U-100 insulin syringe has markings where 100 units equals 1 mL. This is the most common syringe used for peptide injections. Each unit mark represents 0.01 mL. They typically come in 0.3 mL (30 unit), 0.5 mL (50 unit), and 1 mL (100 unit) sizes.

1 mg = 1,000 mcg. To convert mg to mcg, multiply by 1,000. To convert mcg to mg, divide by 1,000. For example, 250 mcg = 0.25 mg, and 2.5 mg = 2,500 mcg.

The amount of water determines the concentration of the solution. More water means a lower concentration (more volume to draw per dose), and less water means a higher concentration (less volume per dose). Our calculator handles the math regardless of how much water you add.

This depends on your desired dose and the concentration of your solution. Use our calculator above -- enter your vial strength, water added, and desired dose, and it will tell you exactly how many units to draw on a U-100 insulin syringe.

You can, but sterile water lacks the preservative (benzyl alcohol) that prevents bacterial growth. With sterile water, you should use the entire vial within 24 hours and never reuse it. Bacteriostatic water allows the reconstituted solution to last up to 30 days.

Store reconstituted peptides in the refrigerator at 2-8 C (36-46 F). Never freeze reconstituted peptides. Keep them away from light. Most reconstituted peptides remain stable for 14-30 days when properly refrigerated, depending on the specific peptide.

Lyophilized means freeze-dried. Peptides are sold in lyophilized (powdered) form because they are much more stable as a dry powder than in solution. Freeze-drying removes water while preserving the peptide's molecular structure. The powder is reconstituted with BAC water before use.

Yes. This calculator uses straightforward mathematical formulas: concentration = vial strength / water volume, and volume to draw = desired dose / concentration. The math is exact. However, real-world accuracy also depends on your syringe precision and technique when drawing the solution.

Each peptide has a unique molecular weight, receptor binding affinity, and pharmacokinetic profile. Some peptides like BPC-157 are active at microgram (mcg) doses, while others like NAD+ require milligram (mg) doses. The doses listed in our database reflect the ranges used in published research.

Draw the desired amount of BAC water into a syringe. Insert the needle through the vial's rubber stopper at an angle. Slowly push the water down the inside wall of the vial -- never spray directly onto the powder. Let the water run gently over the lyophilized cake. Then swirl gently (never shake) until fully dissolved.

Most peptides dissolve within seconds to a few minutes. If there are undissolved particles, gently roll the vial between your palms. Never shake vigorously as this can damage the peptide. If it still does not dissolve after 10-15 minutes of gentle swirling, the peptide may be degraded.

Some peptides can be safely combined in the same syringe for convenience (e.g., BPC-157 + TB-500, or CJC-1295 + Ipamorelin). However, you should never mix peptides in the same vial for storage. Draw each separately into the syringe and inject together.

Ready to Calculate Your Dose?

Scroll up to the calculator or choose a specific peptide calculator for pre-loaded defaults and peptide-specific information.

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